Kuantifikasi Inversi Galaktomanan Kolang-Kaling (Arenga pinnata Merr.) dengan Iodometri
DOI:
https://doi.org/10.52622/jisk.v5i1.03Kata Kunci:
Kolang-Kaling, galactomannan, chromatography, iodometryAbstrak
Background: Kolang-Kaling (Arenga pinnata Merr.) belongs to the Arecaceae family as a source of natural polysaccharides in the form of galactomannan which is usually used as an additional ingredient. Objective: The study was aimed at determining whether galactose-mannose from Kolang-Kaling (GKK) could be separated using chromatography and its levels using the iodometry method. Method: Extraction of galactomannan using distilled water as a solvent. Purification by Paper Chromatography using WEB [Water: Ethanol: Butanol] mobile phase; WAB [Water: Acetate: Butanol]; CH3COOH 10.0% and 50.0%; CA 10.0% and 20.0%. Purification by TLC uses a variation of the EA: H mobile phase. Results: The GKK extraction yield was 18,5 g (3.7%). The levels before and after inversion were 85.53% and 90.23% with a galactose-mannose standard of 94.52%. Conclusion: Galactomannan can be obtained from Kolang-Kaling extraction and its levels are determined using the iodometry method
Unduhan
Referensi
E. G. Azero and C. T. Andrade, “Testing Procedures for Galactomannan Purification,” Polym. Test., vol. 21, no. 5, pp. 551–556, 2002.
L. M. Nwokocha, “Galactomannans,” in Handbook of Hydrocolloids, Elsevier, 2021, pp. 273–293.
N. K. Mathur, Industrial Galactomannan Polysaccharides, vol. 3. Boca Raton: CRC Press Taylor & Francis Group, 2012.
H. N. Lavudi and S. Suthari, “Application of Legume Seed Galactomannan Polysaccharides,” Sustain. Agric. Rev. 45 Legum. Agric. Biotechnol. Vol 1, pp. 97–113, 2020.
G. C. G. Martínez-Avila, M. A. Aguilar-Gonzalez, M. L. Chávez-Gonzalez, D. K. Verma, H. Khan, and C. N. Aguilar, “Galactomannan: a Biodegradable Polymer Used for Bio-based Edible Coating and Film Materials,” in Applications of Biodegradable and Bio-Based Polymers for Human Health and a Cleaner Environment, Apple Academic Press, 2021, pp. 223–238.
A. K. Nasution, A. Azhari, S. Suryati, I. Ishak, and Z. A. Nasrul, “Ekstraksi Galaktomanan Dari Ampas Kelapa,” Chem. Eng. J. Storage, vol. 1, no. 3, pp. 10–16, 2021.
A. Candra, Film Pelapis Kitosan-Pati Biji Aren (Arenga pinnata) sebagai Kemasan Fillet Ikan Salmon. Pekalongan: NEM, 2022.
F. Ihsan, F. T. Pertanian, and U. Andalas, “Pembuatan Nori dengan Pemanfaatan Kolang-Kaling sebagai Bahan Substitusi Rumput Laut Jenis Eucheuma cottonii,” Universitas Andalas, 2016.
Y. Liu, F. Lei, L. He, W. Xu, and J. Jiang, “Comparative Study on The Monosaccharides of Three Typical Galactomannans Hydrolyzed by Different Methods,” Ind. Crops Prod., vol. 157, p. 112895, 2020.
F. Rashid, S. Hussain, and Z. Ahmed, “Extraction Purification and Characterization of Galactomannan from Fenugreek for Industrial Utilization,” Carbohydr. Polym., vol. 180, pp. 88–95, 2018.
D. Darmawati, S. Bahri, and H. Sosidi, “Analisis Kadar Glukomanan dari Biji Durian (Durio zeibethinus Murr) dengan Metode Spektrofotometri pada Berbagai Waktu dan Suhu Hidrolisis,” Kovalen J. Ris. Kim., vol. 6, no. 2, pp. 158–164, 2020.
N. Rahim, A. R. Li, D. Kamarun, and M. R. Ahmad, “Isolation and Characterization of Galactomannan from Seed of Leucaena leucocephala,” Polym. Bull., vol. 75, pp. 2027–2037, 2018.
H. A. Pawar and K. G. Lalitha, “Isolation, Purification and Characterization of Galactomannans as an Excipient from Senna tora Seeds,” Int. J. Biol. Macromol., vol. 65, pp. 167–175, 2014.
J. F. Silva Neto et al., “Extraction, Purification and Electrical Characterization of Gross Galactomannan and Purified Galactomannan Obtained from Adenanthera pavonina L. Seeds,” Chem. Biodivers., vol. 20, no. 2, p. e202200888, 2023.
S. Sudarmadji, B. Haryono, and Suhardi, Prosedur Analisa untuk Bahan Makanan dan Pertanian. Yogyakarta: Liberty, 1997.
Unduhan
Diterbitkan
Terbitan
Bagian
Lisensi
Hak Cipta (c) 2024 Jurnal Indah Sains dan Klinis
Artikel ini berlisensiCreative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
This work is licensed under a 
