Quantification of Galactomannan Inversion of Kolang-Kaling (Arenga pinnata Merr.) by Iodometry

Authors

  • Nilsya Febrika Zebua Zebua Fakultas Farmasi, Universitas Tjut Nyak Dhien, Medan-Indonesia Author
  • Sumardi Fakultas Farmasi, Institut Kesehatan Medistra Lubuk Pakam, Indonesia Author
  • Suprianto Fakultas Farmasi, Institut Kesehatan Medistra Lubuk Pakam, Indonesia Author
  • Putri Addina Fakultas Farmasi, Universitas Tjut Nyak Dhien, Medan-Indonesia Author

DOI:

https://doi.org/10.52622/jisk.v5i1.03

Keywords:

Kolang-Kaling, galactomannan, chromatography, iodometry

Abstract

Background: Kolang-Kaling (Arenga pinnata Merr.) belongs to the Arecaceae family as a source of natural polysaccharides in the form of galactomannan which is usually used as an additional ingredient. Objective: The study was aimed at determining whether galactose-mannose from Kolang-Kaling (GKK) could be separated using chromatography and its levels using the iodometry method. Method: Extraction of galactomannan using distilled water as a solvent. Purification by Paper Chromatography using WEB [Water: Ethanol: Butanol] mobile phase; WAB [Water: Acetate: Butanol]; CH3COOH 10.0% and 50.0%; CA 10.0% and 20.0%. Purification by TLC uses a variation of the EA: H mobile phase. Results: The GKK extraction yield was 18,5 g (3.7%). The levels before and after inversion were 85.53% and 90.23% with a galactose-mannose standard of 94.52%. Conclusion: Galactomannan can be obtained from Kolang-Kaling extraction and its levels are determined using the iodometry method

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Published

30-04-2024

How to Cite

[1]
N. F. Z. Zebua, Sumardi, Suprianto, and Putri Addina , Trans., “Quantification of Galactomannan Inversion of Kolang-Kaling (Arenga pinnata Merr.) by Iodometry”, jisk, vol. 5, no. 1, pp. 14–19, Apr. 2024, doi: 10.52622/jisk.v5i1.03.

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