Quantification of Vitamin C in Petai Cinai (Leucaena leucocephala) using UV-Vis Spectrophotometry

Authors

  • Asti Pratiwi Fakultas Farmasi, Institut Kesehatan Medistra Lubuk Pakam, Indonesia Author
  • Aminah Syarifuddin Fakultas Farmasi, Institut Kesehatan Medistra Lubuk Pakam, Indonesia Author
  • Cucu Arum Dwi Cahya Fakultas Farmasi, Institut Kesehatan Medistra Lubuk Pakam, Indonesia Author
  • Sri Rahayu Fakultas Farmasi, Institut Kesehatan Medistra Lubuk Pakam, Indonesia Author

DOI:

https://doi.org/10.52622/jisk.v4i3.01

Keywords:

Petai Cina, spectrophotometry,, vitamin C

Abstract

Background: Vitamin C is very soluble in water and is always used for additional nutrition in the form of supplements. This vitamin is needed by the body because it can increase the body's ability to fight disease and function as a neutralizer of radical particles found in the body, usually referred to as antioxidants. One of them is the Petai Cina plant which is used by the community as a savory flavor in dishes, and can also be used as traditional medicine. Objective: To determine the presence and levels of vitamin C in Petai Cina in the Lubuk Pakam regional market. with quality and quantity analysis. Method: Samples were analyzed qualitatively using a specific color reagent for vitamin C and quality was determined using UV spectrophotometry. Results: The standard vitamin C wavelength is 224.5 nm at a concentration of 4 ppm with the regression equation y = 0.0339x + 0.3922. Qualitatively, it is proven that there is vitamin C in Petai Cina with a quantity of 0.1095 mg/mL. Conclusion: Vitamin is found in Petai Cina with a quantity of 0.1095 circulating in the Lubuk Pakam market

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References

H. Rivai, Petai Cina (Leucaena leucocephala): Penggunaan Tradisional, Fitokimia, dan Aktivitas Farmakologi. Yogyakarta: Deepublish, 2021.

S. Dalimartha, Atlas Tumbuhan Obat Indonesia, vol. 2. Jakarta: Niaga Swadaya, 2000.

N. Fauziyah, “Efek Antiinflamasi Ekstrak Etanol Daun Petai Cina (Leucaena Glauca, Benth) pada Tikus Putih Jantan Galur Wistar,” Universitas Muhammadiyah Surakarta, 2008.

B. Sarwono, Usaha Membuat Tempe dan Oncom. Jakarta: PT Niaga Swadaya, 2010.

M. C. W. Bunganaen, I. N. Sasputra, and I. M. Artawan, “Studi Komparatif Efektivitas Pemberian Ekstrak Daun Lamtoro (Leucaena Leucocephala) dan Salep Gentamisinterhadap Penyembuhan Luka Sayat Kulit Mencit (Mus Musculus),” Cendana Med. J., vol. 8, no. 1, pp. 512–520, 2020.

T. Teonanda, M. R. Ghozaly, and I. M. Abna, “Aktivitas Antibakteri Ekstrak Etanol Biji Tanaman Petai Cina (Leucaena leucocephala (Lam.) de Wit) Terhadap Bacillus subtilis ATCC 6633,” Sainstech Farma J. Ilmu Kefarmasian, vol. 17, no. 1, pp. 9–15, 2024.

A. A. Pradana, “Uji Aktivitas Antioksidan dari Ekstrak Etanol Biji Petai Cina (Leucaena leucocephala (Lam) de Wit) dengan Metode DPPH,” Politeknik Harapan Bersama, 2021.

N. Cholis, Ensiklopedia Obat-Obatan Alami. Semarang: Alprin, 2020.

A. Fauzi, Aneka Tanaman Obat dan Khasiatnya. Yogyakarta: Media Pressindo, 2019.

M. P. Putri and Y. H. Setiawati, “Analisis Kadar Vitamin C pada Buah Nanas Segar (Ananas comosus (L.) Merr) dan Buah Nanas Kaleng dengan Metode Spektrofotometri UV-Vis,” J. Wiyata Penelit. Sains dan Kesehat., vol. 2, no. 1, pp. 34–38, 2017.

Suprianto, I. Hafiz, H. Faisal, and H. M. Harefa, “Validasi Metode Penentuan Tablet Allopurinol Menggunakan Spektrofotometri Ultraviolet dalam Larutan Asam,” J. Kim. Sains dan Apl., vol. 22, no. 2, pp. 29–37, 2019, doi: 10.14710/jksa.22.2.29-37.

E. Yunita, E. N. Arifah, and V. F. Tamara, “Validasi Metode Penetapan Kadar Vitamin C Kulit Jeruk Keprok (Citrus reticulata) secara Spekteofotometri UV-Vis,” Pharm. J. Farm. Indones. (Pharmaceutical J. Indones., vol. 16, no. 1, pp. 118–131, 2019.

M. Tahir, N. Hikmah, and R. Rahmawati, “Analisis Kandungan Vitamin C dan β-Karoten dalam Daun Kelor (Moringa Oleifra Lam.) dengan Metode Spektrofotometri Uv–vis,” J. Fitofarmaka Indones., vol. 3, no. 1, pp. 135–140, 2016.

Suprianto, D. Syamsul, and M. D. Harfiansyah, “Aplikasi Metode Penetapan Kadar Rutin Parasetamol PT. Kimia Farma, Tbk secara HPLC pada Sediaan Tablet Generik dan Bermerek di Medan,” J. Indah Sains dan Klin., vol. 2, no. 1, pp. 1–5, 2020, doi: 10.52622/jisk.v1i1.1.

S. Suprianto, “Optimization of Mobile Phase for Simultaneous Determination of Sweeteners, Preservatives and Dyes by UFLC,” Int. J. ChemTech Res., vol. 11, no. 1, pp. 56–63, 2018.

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Published

31-12-2023

How to Cite

[1]
Asti Pratiwi, Aminah Syarifuddin, Cucu Arum Dwi Cahya, and Sri Rahayu , Trans., “Quantification of Vitamin C in Petai Cinai (Leucaena leucocephala) using UV-Vis Spectrophotometry”, jisk, vol. 4, no. 3, pp. 1–5, Dec. 2023, doi: 10.52622/jisk.v4i3.01.

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