Potential Antioxidant Activity of Dry and Fresh Ethanol Extract of Mahkota Dewa Leaves (Phaleria macrocarpa)
DOI:
https://doi.org/10.52622/jisk.v3i2.68Keywords:
Antioxidant, Phaleria macrocarpa, 1,1-diphenyl2-picrihidraczil, IC50Abstract
Compounds that function as free radical scavengers are known as antioxidants, which inhibit the oxidation process of chemical reactions and work by taking an electron to reduce the negative effects of these free radicals. The Mahkota Dewa plant (Phaleria macrocarpa), a member of the Thymelacaccae family, is known to have potential as a natural antioxidant.Various treatments prior to the extraction process of the Mahkota Dewa leaf affect the level of antioxidant activity was observed to have an effect on Both groups of leaves were extracted by maceration after part of the leaves were air-dried and partially extracted from fresh leaves. Yields were determined and phytochemical and antioxidant tests used the 1,1-diphenyl-2-picryhydrazyl method and gallic acid as a comparison. Test results showed yield values ??of 13.6% w/w (dried extract) and 9% w/w (fresh leaves). Mahkota dewa leaves contain alkaloids, flavonoids and phenolic active compounds. An antioxidant activity (IC50) value was obtained at 108.05 ppm (dried leaves). 90.97 ppm (fresh leaf) and 4.04 ppm (comparison compound). Fresh leaf sample extracts have higher antioxidant activity values than dried leaf sample extracts.
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References
Winarsi H. Antioksidan Alami & Radikal Bebas.Yogyakarta: Kanisisu. 2007.
Soeksmanto A, Hapsari Y, Simanjuntak P. Kandungan Antioksidan pada Beberapa Bagian Tanaman Mahkota Dewa, P. macrocarpa (Scheff) Boerl.(Thymelaceae). Jurnal Biodiversitas. 2007;8(2):92–5.
Idris NA. Uji Aktivitas Antioksidan Ekstrak Sarang Lebah dan Madu Hutan dari Luwu Utara dengan Metode DPPH (1, 1-difenil-2-pikrilhidrazil). Universitas Islam Negeri Alauddin Makassar; 2017.
Sayuti K, Yenrina R. Antioksidan Alami dan Sintetik. Padang Universitas Adalas. 2015;40.
Surya A, Rahayu DP. Antioksidan Ekstrak Metanol Kulit Petai (Parkia speciosa Hassk) dengan Metode 2, 2-diphenyl-1-picrylhidrazyl. JOPS (Journal Of Pharmacy and Science). 2020;3(2):1–5.
Kristiningrum N, Hernawati S, Aulia RP, Wardani P. Studi Aktivitas Antioksidan Kombinasi Ektrak Etanol Daun Mangga Bachang (Mangifera foetida Lour.) dan Kelopak Bunga Rosella (Hibiscu sabdariffa L.). In Prosiding Seminar Nasional Pendidikan Biologi dan Saintek Ke-3; 2018.
Wahab MF, Indahsari Y, Nurdiana AMM, Nur PBA. Uji Aktivitas Antimikroba Ekstrak Daun Mahkota Dewa (Phaleria macrocarpa) dengan Metode Difusi Cakram. Indonesian Journal of Fundamental Sciences Vol. 2020;6(1).
Desmiaty Y, Ratih H, Dewi MA, Agustín R. Penentuan Jumlah Tanin Total pada Daun Jati Belanda (Guazuma ulmifolia Lamk) dan Daun Sambang Darah (Excoecaria bicolor Hassk.) secara Kolorimetri dengan Pereaksi Biru Prusia. Ortocarpus. 2008;8(1):106–9.
Verawati V, Nofiandi D, Petmawati P. Pengaruh Metode Ekstraksi terhadap Kadar Fenolat Total dan Aktivitas Antioksidan Daun Salam (Syzygium polyanthum (Wight) Walp.). Jurnal Katalisator. 2017;2(2):53–60.
Depkes RI. Farmakope Herbal Indonesia. Jakarta: Departemen Kesehatan Republik Indonesia. 2008;
Mahapatra AK, Nguyen CN. Drying of Medicinal Plants. In: Acta Horticulturae [Internet]. International Society for Horticultural Science (ISHS), Leuven, Belgium; 2007. p. 47–54. Available from: https://doi.org/10.17660/ActaHortic.2007.756.5
Imadahidayah T. Perbandingan Aktivitas Antioksidan Ekstrak Etanol 70% Daun Binahong (anredera cordifolia,(ten.) Steenis) Segar dan Daun Binahong (anredera cordifolia,(ten.) Steenis) Kering dengan Metode DPPH (2, 2-diphenil-1-picryl-hydrazyl). Jurnal Farmasetis. 2015;4(2):51–7.
Yulianingtyas A, Kusmartono B. Optimasi Volume Pelarut dan Waktu Maserasi Pengambilan Flavonoid Daun Belimbing Wuluh (Averrhoa bilimbi L.). Jurnal Teknik Kimia. 2016;10(2):61–7.
Molyneux P. The Use of The Stable Free Radical Diphenylpicrylhydrazyl (DPPH) for Estimating Antioxidant Activity. Songklanakarin J sci technol. 2004;26(2):211–9.
Olatunya AM, Akintayo ET. Evaluation of The Effect of Drying on The Chemical Composition and Antioxidant Activity of The Essential Oil of Peels from Three Species of Citrus Group. International Food Research Journal. 2017;24(5):1991–7.
Shahidi F. Natural Aantioxidants: Chemistry, Health Effects, and Applications. The American Oil Chemists Society; 1997.
Parakash A, Rigelhof F, Miller E. Antioxidant Activity, Medallion Laboratories Analytical Progress. 2001;
Putranti RI. Skrining Fitokimia dan Aktivitas Antioksidan Ekstrak Rumput Laut Sargassum duplicatum dan Turbinaria ornata dari Jepara. Universitas Diponegoro; 2014.
Sutriningsih S. Uji Aktivitas Antioksidan dengan Metode DPPH Ekstrak Daun Katuk (Sauropus Androgynus (L.) Merr) Serta Uji Stabilitas Pengaruh Konsentrasi Emulgator Asam Stearat dan Trietanolamin terhadap Formulasi Krim. Indonesia Natural Research Pharmaceutical Journal. 2018;3(1):119–30.
Stankovic MS. Total Phenolic Content, Flavonoid Concentration and Antioxidant Activity of Marrubium peregrinum L. extracts. Kragujevac J Sci. 2011;33(2011):63–72.
Luginda RA, Sari BL, Indriani L. Pengaruh Variasi Konsentrasi Pelarut Etanol terhadap Kadar Flavonoid Total Daun Beluntas (Pluchea indica (L.) Less) dengan Metode Microwave–Assisted Extraction (MAE). Jurnal Online Mahasiswa (JOM) Bidang Farmasi. 2018;1(1).
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